Thank you for submitting your Paper of the Quarter application. Your submission is summarised below.
Quarter
Q2, 2025
Applicant
Masako Kaufmann
Paper Title
Effective genome editing with an enhanced ISDra2 TnpB system and deep learning-predicted ωRNAs
The name(s) of the first, last or corresponding authors who are DG-GT members
Kim Fabiano Marquart
Paper Link / Upload
Date of the online publication
10 April 2025
Summary
optimized the design of Deinococcus radiodurans (ISDra2) TnpB for application in mammalian cells (TnpBmax), leading to an average 4.4-fold improvement in editing. In addition, we developed variants mutated at position K76 that recognize alternative target-adjacent motifs (TAMs), expanding the targeting range of ISDra2 TnpB. We further generated an extensive dataset on TnpBmax editing efficiencies at 10,211 target sites. This enabled us t
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